Journal: bioRxiv

Article Title: Small molecule inhibition of the mitochondrial lipid transfer protein STARD7 attenuates influenza viral replication

doi: 10.64898/2026.01.20.700505

Figure Lengend Snippet: The M4 compound is a broadly-acting influenza virus inhibitor: ( A ) Chemical structure of M4. (B) After pretreatment with M4 for 16h at the indicated concentrations, A549 cells were infected with influenza A/WSN/33 virus at MOI of 0.1, (C) MEF cells were infected with at MOI of 0.5 and (D) HTBE cells were infected at MOI of 0.25. 48h post infection, cells were fixed, stained for NP, and analyzed with a high content immunofluorescence imager. Percent infection was calculated as the ratio of anti-NP-stained cells to DAPI stained cells. Data are normalized by the mean for DMSO-treated wells and are shown as means ± SEM from three independent experiments. Dose-response curves for infectivity (black) and cell number (red) are shown. (E) MDCK cells were pre-treated for 16hs with different concentrations of M4 followed by infection with the indicated influenza A subtypes at MOI of 0.1 or influenza B/Yamagata at MOI of 0.2. Supernatants were analyzed at 24h post infection by plaque assay and data are represented as means ± s.d. of three independent experiments. The IC 50 values are indicated in .

Article Snippet: HTBE cells (ATCC PCS-300-010) were cultured in commercially available airway epithelial cell basal media following the manufacturer’s protocol (ATCC).

Techniques: Virus, Infection, Staining, Immunofluorescence, Plaque Assay

Journal: Cancer Cell International

Article Title: Circulating extracellular vesicle PTPRO methylation: an exploratory biomarker for minimally invasive diagnosis of early-stage lung adenocarcinoma

doi: 10.1186/s12935-025-04127-9

Figure Lengend Snippet: Methylation analysis of PTPRO in LUAD cell lines and LUAD cells derived sEVs. (A) The mRNA expression levels of PTPRO were determined by RT-qPCR in the normal lung epithelial cell line HBE and LUAD cell lines A549, NCI-H460, and PC-9. (B-C) PTPRO methylation status in HBE and the LUAD cell lines A549, NCI-H460, and PC-9 was assessed using MSP and q-MSP assays. (D) Representative TEM images of sEVs purified from the conditioned media of HBE and LUAD cell lines (A549, NCI-H460, and PC-9). Scale bar: 100 nm. (E) The concentration and size distribution of sEVs from HBE and LUAD cell lines (A549, NCI-H460, and PC-9) were evaluated using NTA. (F) Expression of sEV markers (TSG101, Alix, CD63, and Calnexin) and PTPRO in sEVs purified from HBE and LUAD cell lines (A549, NCI-H460, and PC-9) was detected by immunoblotting. (G) The mRNA expression levels of PTPRO in sEVs purified from the conditioned media of HBE and LUAD cell lines (A549, NCI-H460, and PC-9) were measured by RT-qPCR. (H-I) MSP and q-MSP analyses of purified sEVs from HBE and LUAD cell lines (A549, NCI-H460, and PC-9). H 2 O was used as a negative control. Error bars indicate SEM. *** P < 0.001 by one-way ANOVA with Dunnett's multiple comparisons test.

Article Snippet: Human LUAD cell lines (A549, PC-9, and NCI-H460) and normal human bronchial epithelial cells (HBE) were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Methylation, Derivative Assay, Expressing, Quantitative RT-PCR, Purification, Concentration Assay, Western Blot, Negative Control